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What Is The Importance Of Contrast In Microscopy?

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Contrast is everything in microscopy. By definition, microscopy involves viewing objects that can't be seen by the naked eye- by applying contrast, these objects come into view and can be observed at close quarters. Contrast is required in the three main areas of microscopy which are optical, electron and scanning probe.

The development of microscopes has been invaluable in a number of fields, allowing students and researchers to study close up, make notes and get inside the topic that they are studying. Microscopes have been generally available since the early 1900s and are now mass produced giving incredible definition and options to study. Students and researchers have been able to follow growth patterns and get inside their subjects using microscopes with the optical microscope by far the most popular in terms of numbers.

Using a combination of lenses to enlarge an object allows the student to see an object at up to 1500 times its normal size. The lenses used are precision built and combined for effect. To make the best use of the microscopes, you have to understand your subject fully, and the type of results that you are looking for. Getting the best results from a microscope is in itself a complex task, it's not a case of simply looking through the viewer and making a couple of adjustments to view the object.

Understanding the combinations of the lenses, how to get to different magnifications and what level of insight that you are needing are all required, before you get to look through the microscope. Balancing the light and contrast with the degree of magnification, will allow you to obtain the best possible results- and to record those results for future study.

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