What Is Random Primer Labelling?


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Aun Jafery answered
Random Primer labelling is a technique that is used in situ hybridization in sections of tissue. Random Primer DNA Labelling is used in the production of DNA strands that are radioactively labelled. This is done to detect RNA or DNA sequences in different applications including those involving northern and southern blot hybridization. The material required for primer labelling requires Gibco BRL Random Premier Labeling Kit, a dsDNA Fragment, [a-³²P]dCTP and distilled water. Additional equipment needed in Random Primer DNA Labeling include equipment for radioactivity monitoring and waste management, a 1 mL syringe and Sephadex G-50 that is saturated in TE buffer pH 7.4 and 0.2 per cent SDS. The step by step procedure to conduct this procedure is available at www.cbs.umn.edu/a>. You need a purified dsDNA fragment of some known concentration to carry on the procedure.

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