Ammonia acetate is a salt formed as a reaction of ammonia and acetic acid. It is a very weak acid and a weak base. It has several distinctive properties and is used for several purposes. It is used as a material used for protein and nucleic acid purifications. This is so because of its solubility in acetone and methanol. It is easy to precipitate proteins or nucleic is such solvents.
Thus this acid is widely used in protein precipitating processes and is a common ingredient in such experiments and procedures due to their solubility. It is used because it has the capability to keep proteins cold which is essential in this process. It is method of purifying proteins but is not considered as the best method to do it because it is not capable of the level required and can provide only up to a few folds of purification. The substances or chemicals that assist this process are also known as buffers. Therefore ammonium acetate is also a buffer.
Thus this acid is widely used in protein precipitating processes and is a common ingredient in such experiments and procedures due to their solubility. It is used because it has the capability to keep proteins cold which is essential in this process. It is method of purifying proteins but is not considered as the best method to do it because it is not capable of the level required and can provide only up to a few folds of purification. The substances or chemicals that assist this process are also known as buffers. Therefore ammonium acetate is also a buffer.
