To map gene, you first isolate the DNA from tissues of your species of interest. First you need to prepare some buffers like lysis buffer, TAE buffer, solution of chloroform phenol iso-amyl alcohol at 25:24:1 ratio.
Not only buffers but you need to homogenize tissues of your interest with a high-speed benchtop homogenizer with further preparation of your gel electrophoresis apparatus to run the sample DNAs with markers.
