What Is Difference Between Chromatography And Electrophoresis?

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What Is Difference Between Chromatography And Electrophoresis?

2 Answers

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Answer 1

Chromatography, like electrophoresis, is a separation technique used by scientists, usually chemists.

In chromatography, there is a mobile phase and a stationary phase. The stationary phase is usually a solid, and the mobile phase is usually a liquid or even a gas. As the mobile phase moves over the stationary phase, it takes some of the components in the mixture along with it, but at different rates. The rate depends on how strongly the mixture components stick to the stationary phase.

In electrophoresis, an electric potential gradient is applied across the stationary phase. There is also a wet mobile phase, usually acidic or basic. Ions in the mixture are pulled across the stationary phase by the electric potential by different amounts, and the species are separated.

The mixture in chromatography is applied at the bottom of the stationary phase and the components move up the phase at different rates. But in electrophoresis, it is applied in the middle of the stationary phase and the components move towards the positive or negative electrode depending on their charge.

Answer 2

Paper chromatography is an analytical technique for separating and identifying mixtures that are or can be colored and agarose gel electronphoresis is a method used in biochemistry and molecular biology to separate DNA, DNA or molecules by size. This is achieved by moving negatively charged nucleic acid molecules through an agarose matrix with an electric field (electrophoresis) shorter molecules move faster and migrate farther than longer molecules.