What Is Paper Chromatography?


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Paper Chromatography basically is an analytical method applied for separating and recognizing complexes which can be coloured, mainly pigments. This procedure has been mainly substituted by thin stratum chromatography; nevertheless it is still a vital teaching tool.

Two-way paper chromatography, also known as two-dimensional chromatography, usually involves using two solvents and turning the paper 90 degrees in between. This is helpful in dividing compound mixtures of identical complexes, like amino acids. In quiet a cases, paper chromatography fails to separate pigments entirely; this takes place when two substances seem to have identical values in a particular solvent. In these circumstances, two-way chromatography is applied to divide the multiple-pigment spots.

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