What Is The Role Of Enzymes In Diagnosis?

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What Is The Role Of Enzymes In Diagnosis?

2 Answers

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Answer 1

Enzymes are used for the diagnosis of diseases, that is, enzymatic diagnosis is a method for determining the content and changes of certain substances in the body by the catalytic action of the enzyme, or for diagnosing the disease by the change of the original enzyme activity in the body.

Besides protein engineering, chemical approaches including chemical modification and immobilization are employed in designing biocatalysts with desirable bio-properties for industrial applications. Enzyme immobilization, first commercialized in the 1960s, has been well developed to facilitate the reusability and recovery of enzymes. High stabilization of a biocatalyst, such as pH and thermal stability, can often be achieved through immobilization, and enhanced activities are demonstrated in an increasing number of recent reports.

Answer 2

The idea is that when organs which produce specific enzymes get injured, enzyme activity decreases in the organ and the concentration of these enzymes increase in the plasma. Thus, biochemical investigations are set up to measure increases in plasma of enzyme activity.So when diagnosing certain conditions like myocardial infarction, the enzyme activity in plasma is measured assuming that all enzymes in the human body are synthesized intracellularly.There are significant quantities in plasma once cells of organs are damaged which are measured.Enzyme activity is normally related to the number of cells damaged and thus to the extent of lesion.Correct interpretation of enzyme activity in disease depends on understanding:
»factors that affect the rate of enzyme release from cells of origin
»the rate at which they are cleared from circulation
Thus professionals have come up with typical graphs of increase in activity of enzymes after certain occasions like myocardial infarction.